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Biophysics Core

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The Biophysics Core Facility supports JHMRI investigators who want to characterize macromolecules or macromolecular complexes using biophysical techniques (isothermal titration calorimetry, dynamic light scattering, circular dichroism and x-ray crystallography).

Facility equipment can detect multimerization or aggregation of proteins, as well as the formation of macromolecular complexes. The equipment can measure the average particle size of molecules in solution and the binding constants for protein-ligand interactions or protein-protein interactions. The secondary structure content of proteins can be determined and compared with changes in structure upon interaction with a ligand. In addition, the atomic resolution three-dimensional structure of macromolecules and complexes can be determined. These capabilities support investigators studying malaria proteins as targets for drug discovery and vaccine design.

AVAILABLE TECHNIQUES

  • Isothermal Titration Calorimetry 
          MicroCal VP-ITC

  • Dynamic Light Scattering
           Proterion DynaPro
           Peltier temperature
           control          

  • Circular Dichroism
           Jasco J-810 Spectropolarimeter
           Peltier temperature control
           Automated dual titration system
           Scanning emission monochromator                                

  • X-ray Crystallography 
            Bruker Microstar x-ray generator 
            with AXS X8 Proteum CCD detector
            Cobra cryocooling device

CONTACT for ITC, DLS and CD:
Jurgen Bosch
jubosch@jhsph.edu

CONTACT for Xray:
Sean Prigge
sprigge@jhsph.edu

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